cd34-pe (ram-34)  (Thermo Fisher)

Journal: Cell Death & Disease

Article Title: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice

doi: 10.1038/s41419-024-06578-w

Figure Lengend Snippet: A Representative z-projection confocal images of CD34 (magenta) in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 50 μm. B Scatter dot plots showing quantification of CD34 signal expressed as the percentual area occupied by fluorescent CD34 staining in GM ( n = 22/5 slices/mice) and GM/ABX ( n = 23/5 slices/mice) mice versus total FOV (top) and number of CD34 + vessel-like structure versus total field of view (bottom) GM ( n = 22/5 slices/mice) and GM/ABX ( n = 23/5 slices/mice). Data are presented as the mean ± SD *** p < 0.001; ** p < 0.01, Student’s t test. C Representative z-projection confocal images of CD34 (magenta) and CD31 (green) colocalization in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 20 μm. D Scatter dot plots showing quantification of CD31 signal expressed as the percentual area occupied by fluorescent CD34 staining in GM ( n = 18/3 slices/mice) and GM/ABX ( n = 18/3 slices/mice) mice versus total field of view (left) and number of CD31 + vessel-like structure versus total field of view (GM n = 18/3 slices/mice; GM/ABX n = 15/3 slices/mice) (right). Data are presented as the mean ± SD * p < 0.05, Student’s t test.

Article Snippet: Cells were stained with the following fluorochrome-conjugated mAbs (clone name indicated in parentheses) for 20 min at 4 °C: CD45.2-APC-eFluor 780 (104), CD11b-APC (M1/70), Tmem119-Alexa Fluor™ 488 (V3RT1GOsz) from eBioscience TM-Invitrogen (Thermo Fisher), CD34-PE (RAM-34) from eBioscience TM .

Techniques: Staining

Journal: Cell Death & Disease

Article Title: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice

doi: 10.1038/s41419-024-06578-w

Figure Lengend Snippet: A Representative z-projection confocal images of CD34 (magenta) and CD133 (green) in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 25 μm; zoom 10 μm. B Scatter dot plots showing quantification of CD133 + positive cells (GM: n = 16/7/3 FOV/slices/mice; GM/ABX: n = 21/8/3 FOV/slices/mice. Data are presented as the mean ± SD * p < 0.05; Student’s t test). C Scatter dot plots showing quantification of CD133 + CD34 + double-positive cells (GM: n = 16/7/3 FOV/slices/mice; GM/ABX: n = 21/8/3 FOV/slices/mice. Data are presented as the mean ± SD * p < 0.05; Student’s t test). D Representative single-plane confocal images of GL261 rfp (magenta) and CD34 (green) in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization. 60x objective (Scale bar: 20 μm; zoom 20 μm). Yellow stars indicate the colocalizing signals. E Scatter dot plots showing the percentage of rfp-CD34 colocalizing signals (GM n = 40/10/3 FOV/slices/mice; GM/ABX n = 40/10/3 FOV/slices/mice). Data are presented as the mean ± SD *** p < 0.001 Student’s t test.

Article Snippet: Cells were stained with the following fluorochrome-conjugated mAbs (clone name indicated in parentheses) for 20 min at 4 °C: CD45.2-APC-eFluor 780 (104), CD11b-APC (M1/70), Tmem119-Alexa Fluor™ 488 (V3RT1GOsz) from eBioscience TM-Invitrogen (Thermo Fisher), CD34-PE (RAM-34) from eBioscience TM .

Techniques: Staining

Journal: Cell Death & Disease

Article Title: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice

doi: 10.1038/s41419-024-06578-w

Figure Lengend Snippet: A Representative z-projection confocal images of CD34 (magenta) and CD45 (green) in the GM tumor core area. Hoechst staining (blu) for nuclei visualization. 60x objective (Scale bar: 25 μm; zoom 20 μm). Yellow stars indicate the CD45 + CD34 + cells. B Flow cytometry quantification of CD45 + CD34 + cells in GM and GM/ABX tumoral hemisphere. C CD45 high CD34 + population identifying the peripheral infiltrating hematopoietic endothelial progenitor cells and ( D ) CD45 int CD34 + identifying the resident brain population expressing the CD34 marker (mostly likely microglia/macrophages). n = 5 mice per group. Data are presented as the mean ± SD.

Article Snippet: Cells were stained with the following fluorochrome-conjugated mAbs (clone name indicated in parentheses) for 20 min at 4 °C: CD45.2-APC-eFluor 780 (104), CD11b-APC (M1/70), Tmem119-Alexa Fluor™ 488 (V3RT1GOsz) from eBioscience TM-Invitrogen (Thermo Fisher), CD34-PE (RAM-34) from eBioscience TM .

Techniques: Staining, Flow Cytometry, Expressing, Marker

Journal: Cell Death & Disease

Article Title: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice

doi: 10.1038/s41419-024-06578-w

Figure Lengend Snippet: A Tumor size in GM and GM/ABX mice with or without GlyT1 inhibitor, n = 5–9, pooled from two experiments. Data are presented as the mean ± SD ** p < 0.01, *** p < 0.001, one-way ANOVA followed by Tukey’s multiple comparison test. B Representative images of brain coronal slices, scale bar = 1 mm. C Representative confocal images of CD34 (magenta) in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 50 μm. D Scatter dot plots showing quantification of CD34 signal expressed as the percentual area occupied by fluorescent CD34 staining in GM ( n = 18/3 slices/mice), GM/ABX ( n = 18/3 slices/mice), GM + GlyT1 inh ( n = 19/3 slices/mice), GM/ABX + GlyT1 inh ( n = 20/3 slices/mice) mice versus total field of view (FOV) Data are presented as the mean ± SD *** p < 0.001; one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Cells were stained with the following fluorochrome-conjugated mAbs (clone name indicated in parentheses) for 20 min at 4 °C: CD45.2-APC-eFluor 780 (104), CD11b-APC (M1/70), Tmem119-Alexa Fluor™ 488 (V3RT1GOsz) from eBioscience TM-Invitrogen (Thermo Fisher), CD34-PE (RAM-34) from eBioscience TM .

Techniques: Comparison, Staining